A next-generation dual guide CRISPR system for genetic ... - Nature
Dec 6, 2025 · Large-scale combinatorial perturbation screens are important to identify genetic interactions and synthetic lethal gene pairs. Here, Burgold et al. present a next-generation dual guide …
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To accelerate precision cancer medicine, we construct systematic key datasets, analytical and visualization tools, using broad panels of cancer models that represent the diversity of human cancers.
A compendium of synthetic lethal gene pairs defined by extensive ...
Sep 18, 2025 · Results We generate a dual-guide CRISPR/Cas9 Library and analyse 472 predicted synthetic lethal pairs in 27 cancer cell Lines from melanoma, pancreatic and lung cancer Lineages. …
A comprehensive clinically informed map of dependencies in cancer cells ...
Feb 12, 2024 · Here, we construct a second-generation map of cancer dependencies by annotating 930 cancer cell lines with multi-omic data and analyze relationships between molecular markers and …
CRISPR: Genome Scale Knockout - The Cancer Dependency Map at …
Processed Data (Cell Lines) Descriptions of how the raw data was processed including algorithms and filtering. Processed datasets can be downloaded here, the active dataset can also be accessed …
Generation of dual-gRNA library for combinatorial CRISPR screening …
Sep 16, 2022 · An overview of workflow chart can be found in Figure 1. Following this protocol, pooled library of 62,500 dual-gRNA combinations for targeting more than 1,000 gene pairs was successfully …
DepMap - Broad Institute
We developed CERES to estimate gene-dependency levels from CRISPR-Cas9 essentiality screens while accounting for this effect. In our efforts to define a cancer dependency map, we performed …
SLKB: synthetic lethality knowledge base - Oxford Academic
Oct 27, 2023 · Abstract Emerging CRISPR–Cas9 technology permits synthetic lethality (SL) screening of large number of gene pairs from gene combination double knockout (CDKO) experiments. However, …
SLKB: synthetic lethality knowledge base - PMC
Emerging CRISPR–Cas9 technology permits synthetic lethality (SL) screening of large number of gene pairs from gene combination double knockout (CDKO) experiments. However, the poor integration …
BioGRID CRISPR Screen Database | BioGRID ORCS
Oct 1, 2025 · The BioGRID ORCS curated CRISPR database has been updated to include CRISPR screens from a total of 418 different publications. These additions bring the total number of curated …
Combinatorial CRISPR–Cas9 screens for de novo mapping of ... - Nature
Mar 20, 2017 · A library of plasmids expressing two gRNAs allows for the mapping of combinatorial genetic interactions with the CRISPR system. Results in cancer cells suggest that cellular context is …